FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1801_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1801_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953840
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC34310.17560291528477254No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT31510.16127216148712278No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG23720.12140195717151865No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC20720.10604757810260819No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT19790.10128772059124595No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC701.693879E-551.7996941
CTCCGAC1351.9099389E-1048.33277145
GACGACT2005.456968E-1239.8857654
ACGACTC2005.456968E-1239.8857655
CGACTCC2151.2732926E-1137.1030356
CACGAGC4500.037.055122145
AGACGAC2302.7284841E-1134.6832733
GCGATTA1051.8544888E-434.523407145
TAATCGT850.00274277934.117259
TTAGGTA8050.030.6293834
AAGACGA2651.382432E-1030.1024632
GTTGACA11200.029.7848221
GTTACGT2003.2493335E-729.0078281
TTACGTG1804.6931127E-628.2020552
TATACTG9000.028.2020555
ATTAGGT10050.025.977163
GATATAC3108.56744E-1025.732753
AGCGAAA9600.025.6840131
ACATGGT13050.025.5624545
GGATTAG13400.024.894781
Produced by FastQC (version 0.11.5)