FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1801_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1801_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087906
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC88920.28796213356235584No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC45510.14738142935698173No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC43580.14113123909859948No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC42350.1371479572240865No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT41710.13507535527312037No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA36590.11849453966539136No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC36180.1171667790405537No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT31890.10327386908798389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC6100.059.42574145
CTCCGAC2450.050.30571145
TCTCCGC11500.042.238785145
GTATTAT26150.034.106581
GTTGACA21200.031.1250691
ACATGGT20200.030.1541235
CCACGAC4100.030.060724145
GCGAAAG8450.030.03422
CGTTTAT4400.029.6636491
AAGGTCG4250.029.0006989
TATACTG21100.028.867935
TTACGTG2054.0383384E-728.2970312
GTAGAAA20550.027.5224762
CAGTGCA17650.027.114652
ATCTATG16250.026.7733461
TATTATC17000.026.4452382
CTTATAT13250.026.2681881
GCACCGT18500.026.2612176
AGCGAAA9700.026.1638151
ATCTAAA53850.026.1228621
Produced by FastQC (version 0.11.5)