FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1800_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1800_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422485
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC4100.030.060526145
TGAGCGC5400.029.536592145
ATAGCGT1050.00770877227.6196048
TCTCCGC8000.027.187092145
CGAAAGC6550.026.5719592
GCGAAAG6650.026.1723791
CTCCGAC1450.001226805724.999624145
TATACTG8950.024.3081465
TGTCGTT1851.8313821E-423.51316145
AGCGAAA6350.022.8407271
GTTGACA8900.022.815061
CTACTGA14800.022.0505646
GCGATCG2704.447622E-621.48116145
GGTTCGA2104.3147817E-420.7147038
GATAGCG2908.254587E-619.999699145
GTGATCG2556.959046E-519.902369
AGACGGT3651.361368E-719.862713145
GTATAAG6300.019.56871
ATTATAC5204.3655746E-1119.5244273
GTATATC3001.1035228E-519.338481
Produced by FastQC (version 0.11.5)