FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1800_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1800_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476110
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1551.4551915E-1146.783231
ATGCGCC750.001483688738.66628145
GTGCGAT1406.611226E-736.2508669
CAATCCG900.003628363632.2229929
ACTACGC2101.438093E-831.0774331
AGCGAAA7150.029.4112761
CTCCGAC1050.007709997727.61877145
CTACGCA2405.2652467E-827.1927532
GCGAAAG8000.026.2863312
GTATAAT6500.024.5432031
TATACTG7550.024.011265
GTTGACA7250.024.0046371
CATCGTT2751.966273E-723.7318552
CGAAAGC9300.022.6118973
GATAGCG2252.6528545E-522.555328145
TCGCACC2903.28213E-722.5043474
CTACTGA17750.022.06066
GTACTAG2003.0999538E-421.7542041
TACAGCC8350.021.7071047
GGTGCGA2404.3638905E-521.1463368
Produced by FastQC (version 0.11.5)