FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1800_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1800_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187882
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11930.1004308508757604No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC3500.047.64243145
ATGCCGG3700.043.107723145
GCTACTA2100.041.4299439
TAGTCGG1303.699406E-739.0381138
TTACGTG1701.8280844E-938.388472
CTCCGAC1157.144281E-637.82575145
GACGACT1553.466448E-837.425324
GTTACGT1752.4265319E-937.2916561
CTACGCA1458.6894033E-735.005582
ACTACGC1458.6894033E-735.005581
TATACTG5900.034.4122625
AGACGAC1707.814015E-834.1230853
GATAGCG2353.45608E-1133.935863145
GTTGACA8650.032.6930731
TAGTACT4050.032.227364
GATATAC2257.912604E-1032.2273563
GCGTTAA1352.142054E-532.223299
GTGCGAT1352.142054E-532.223299
TGCGTTA1352.1426706E-532.221938
GTTATGT9800.031.076381
Produced by FastQC (version 0.11.5)