FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1800_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1800_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466007
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA58770.23832049138546646No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT56340.2284665047585023No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA48400.19626870483336017No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA43390.17595246079998964No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG42480.17226228473804006No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG39870.16167837317574524No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA36500.1480125563309431No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT33070.13410343117436407No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC26230.10636628363179829No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCG11250.048.977379
GTAGAAA27500.046.1453362
AGCGAAA13950.037.4266321
TACTGAT10250.036.7801787
GCGAAAG14900.036.0137142
ACTACGC2451.8189894E-1235.517111
GGTCGTT10750.035.0694737
TATACTG34900.034.4909485
CTACGCA2551.8189894E-1234.124282
CGAAAGC16550.032.861373
GTTACGT1801.2915007E-732.2284851
CACGAGC5400.032.22195145
TATTCAA31100.031.9435065
ACAAGGT19350.031.4726037
TAGAAAC41600.031.376293
GTCGTTT11800.031.3344848
GTACTGA13000.031.2368416
CCTTATA32700.030.8240342
ACAAGGC29000.030.7497438
GGTACTG12450.030.2870145
Produced by FastQC (version 0.11.5)