FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1800_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1800_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953547
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10530.11042979528014873No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA10460.10969569407695688No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT10060.1055008300587176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG400.00605100854.3745279
ACTACGC1002.7302285E-643.5087471
CTCCGAC700.001056661641.42821145
AGACCGC2350.040.106033145
CTACGCA1157.1325067E-637.8336942
GTATTAT8550.037.3174441
GCGAAAG2750.036.9165152
CCGACTA1001.3897558E-436.257294
AGCGAAA3000.033.8401371
TATGGAC3200.031.725135
AGGGCGT1153.1621507E-431.521467145
GTTGACA6700.031.3869061
ACATGGT6700.031.3869065
CTTATAT4000.030.8186971
TATATTG6150.029.477475
CGAAAGC3450.029.4262073
CGACTCC1000.00606835229.0058336
CACCGTA1000.006074575228.999757
CACGAGC3102.7284841E-1128.064274145
GTATATA3650.027.8138121
Produced by FastQC (version 0.11.5)