FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1797_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1797_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520734
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC17400.11441843215184247No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG15780.10376568157218816No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG15600.10258204261889324No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG803.735887E-545.311863145
GCGAAAG6900.044.1428762
AGCGAAA6900.043.0918541
GGTACTG7350.036.508095
CGAAAGC9100.035.8617553
GGATAGC7850.031.411165
GAACCGC1703.0056926E-629.8623396
ACTGATC11000.029.0005468
GTACTGA10100.028.0038626
TATAACG2853.2196112E-1027.9912665
AACCGCG1306.482554E-427.8860597
TACTGAT10400.027.8860597
CACGAGC4150.026.20445145
TCGTCGA1409.995895E-425.8933459
AGGTACT10500.025.5556624
ATACCGT1450.001224487925.0078726
TAACGTC3301.7735147E-924.1679177
CCGACTA1851.827373E-423.5209184
GATAGCA10700.023.0446386
TGGATAG10400.023.012054
Produced by FastQC (version 0.11.5)