FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1797_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1797_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639453
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT26680.16273720564114982No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA17810.10863379432042274No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTA400.006052041454.373936145
AGCGAAA7050.041.149011
GCGAAAG7500.039.6470682
CGAAAGC8100.036.7102473
TATACTG9300.035.872645
GTCCGTA1000.006065329.0100481
TATAACG3357.4578566E-1125.9791495
CGACGGC351.2569712E-424.856655105-109
CACGAGC3301.7753337E-924.166193145
GTAGAAA10950.023.8438762
GCTATAT5000.023.2080381
TATACTA2852.771776E-722.902675
GTACTAG1600.00217136222.6641031
ACCTTAT13800.022.5984081
AACGTCC3854.274625E-1022.598348
AATATAC15550.021.9207783
GTATTAT20550.021.881061
GTAATAT11300.021.8217181
CGCTGCC4109.404175E-1021.219095145
GTATAGA15400.021.1924081
Produced by FastQC (version 0.11.5)