FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1797_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1797_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332468
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG142710.611841191390407No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC122500.5251947722326737No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC81510.3494581704872264No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG76430.32767866483055713No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT69890.2996396949497271No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG60940.26126832179476844No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC60070.2575383670858507No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA58400.25037856896643385No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG54630.23421543189445687No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC50080.21470819749724326No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT46120.19773047261527274No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA45200.1937861526931988No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG43680.1872694502132505No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC43080.1846970676553762No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA40760.17475052176492883No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC39810.17067758271496114No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC38840.16651889757973098No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC37990.1628746889560757No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC37070.15893036903400176No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG35490.15215642829826606No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA35420.15185631699984736No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG33060.14173827893887506No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA32130.13775108597416985No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG31830.13646489469523268No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG31400.13462135386208943No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG30670.131491621750009No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA30540.1309342721958029No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA29660.1271614444442539No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC29330.125746634037423No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT27870.11948716981326217No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT27740.11892982025905607No Hit
CTAATAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTG26610.1140851664417261No Hit
TTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCAT26590.11399942035646364No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTACAACTCG26570.11391367427120115No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT26540.11378505514330743No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA26470.11348494384488877No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA25980.11138416475595805No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT25720.11026946564754586No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC25610.10979786217860223No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT24580.10538193878758466No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT24340.10435298576443493No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT23720.10169485712129814No Hit
CATTTATGGTATAGCTCGAAACAGCCTTTCCCATCTTCCATAGCATTGGA23600.10118038060972327No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG23520.10083739626867336No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG23520.10083739626867336No Hit
CATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTACTACTC23440.10049441192762346No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA23440.10049441192762346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA16000.0114.198892
GGTACTG16100.0111.690555
GACTTAA553.6379788E-12105.45374145
CACGAGC900.0104.72143145
CCCACGC700.093.213585145
GTACTGA21550.086.8086556
CAGGTAC20950.086.178033
AACACTG11800.081.111515
AGGTACT23450.080.702734
CTAGATA451.2334585E-680.55495145
CGAGACA1050.075.951805145
AGTAGAA13900.074.072131
CCACGAC955.456968E-1268.68369145
GACTCGC754.8585207E-967.66616145
CGCAATT902.8194336E-1064.443954145
ATATCAC5550.058.7896423
CTTATAA2100.058.690033145
AAAGCGC502.0108769E-457.99956145
AAATATA2300.056.7460025
CAGGGTG1450.055.006665
Produced by FastQC (version 0.11.5)