FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1797_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1797_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465459
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG185580.752719878935322No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT65190.2644132390763748No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC57160.23184323892630135No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG55160.22373115918780237No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT52040.2110763147957439No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA49970.20268031226639746No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT45300.1837386060770023No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT42140.1709215200901739No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG41740.1692991041424741No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA40910.16593259105099697No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT40270.16333672553467732No Hit
GTTTAGAAATGTCTTGAGCATTGCCCCTATAATGTTCTCAAATAAAATGG38830.15749602812295804No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT37140.1506413207439264No Hit
ATATTGGCTTCTGGCAGCCTTTGGTACTAAAGATTGAAATGGTTCAAACT35990.14597687489428945No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT35490.14394885495966472No Hit
CCTTTACAGTTACCGGAGACAACACCAAATGGAATGAGAATCAGAATCCT34450.13973057349564524No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG34350.13932496950872028No Hit
CTTATTAGGTAGCTCTTTTTTGTCAGCTTTTGCTTCTTCTTCCCTATTGT34100.1383109595414079No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG33760.13693190598586308No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA33640.13644518120155313No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC33020.13393043648261846No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA32110.13023944020160141No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT31880.12930655103167402No Hit
CCATAGAGGTATTCAGATCGAACGGTCTAACAGCCAATGAGTCAGGAAGG31590.12813029946959165No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG30990.12569667554804198No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG30040.12184343767225495No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC29290.11880140777031781No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC29150.1182335621886229No Hit
ATATAGAAGTATTGCATTTGACTCAAGGAACTTGCTGGGAGCAGATGTAC28680.11632722345007562No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC28550.1157999382670732No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG28520.1156782570709957No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA28420.11527265308407075No Hit
CCTATAATGTTCTCAAATAAAATGGCGAGGTTAGGAAAAGGATACATGTT28250.11458312630629834No Hit
TCTATGATGTGGGAGATTAACGGGCCAGAATCAGTGCTAGTTAACACATA28220.11446144511022086No Hit
GTAAGGGAGAATTCCATACTCACAAAGTTCACCACGTCAGTATCATTTCT27770.11263622716905858No Hit
ATAATAAGATTGATGACCAAATACAAGACGTATGGGCATATAATGCAGAA27750.1125551063716736No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA26780.10862074769850158No Hit
AGTTAACACATATCAATGGATCATTAGGAATTGGGAGACTGTAAAGATCC26490.10744449613641921No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC26420.10716057334557176No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC26370.10695777135210928No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT26130.1059843217834894No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG25770.10452414743055959No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC25480.10334789586847722No Hit
CAATGAGGCTGTGCCCTCTATTAGTAGAGGTCTTATTTTCTCAATTTTCT25440.10318565427370725No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA25220.10229332550247235No Hit
GGTTAATAGATTTCCTCAAGGACGTAATGGAATCAATGGATAAGGAAGAA25030.10152267792731497No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA24930.10111707394039002No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT24900.10099539274431252No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG151.2288988E-4145.039185
GCAGGTA4550.095.630242
GGTACTG4750.091.60375
CCCACGC1200.090.62375145
AGTAGAA7100.082.733621
CTCCGAC1100.072.49901145
CGAGTCA300.001936185572.499145
CACGAGC1800.072.499145
AGGTCGG300.001936185572.499145
AACACTG7300.067.55255
CGACAGG350.00356731862.142002145
ATATCAC3050.061.8199843
AGGTACT7300.061.5919884
CTTATAA2850.061.051792145
GTACTGA8300.059.4136436
CCGTGTG1102.5465852E-1159.3342175
TATAGCG9100.054.987392
GTTATGG400.00604546754.3896983
ATCACTA3750.052.214115
GCGGTAT9650.051.8533866
Produced by FastQC (version 0.11.5)