FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1797_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1797_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084721
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT30420.14591880640143212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC6250.053.359386145
GCGAAAG6550.046.498052
AGCGAAA7600.040.0720521
CGAAAGC9000.034.6459663
ACCGTAC1601.8761202E-631.7237071
TATACGG1000.00606988829.0059265
GCGTATA2354.2957254E-827.7702981
GTATAGG10750.026.980961
CTCCGAC2259.116393E-725.77748145
TATACTA10650.023.150275
GGATAGC6300.023.0205765
GAGCGAA2903.28464E-722.5035171
TATACTG14950.022.312255
TACGGAC2303.1391777E-522.0697254
GTAGGAC12550.021.9566763
TATATAG24950.021.798043
CTATACT16650.021.3406964
TATACCG8600.021.0798875
GATTGTT23200.020.313249
TAGTCGG2155.056606E-420.233788
Produced by FastQC (version 0.11.5)