FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1796_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1796_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563697
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7400.046.057111
GCGAAAG7750.045.848482
CGAAAGC10550.032.9928133
CTACGGA1652.3836E-630.764133
TTACGAG2054.034082E-728.298713
GTAGACC3700.027.4382783
TACACCG2156.116916E-726.9824925
GACCGAT2156.1288847E-726.9764547
TAGGACT9350.026.3692534
GCACCGT3657.2759576E-1225.827426
GTCCGTA1751.2543522E-424.862441
TCCGTAG1751.2543522E-424.862442
CCGTAGA2401.5960813E-624.1718163
GTGTAGG10200.023.4608821
TACGGAC2252.6484146E-522.5603624
TCTACGG2303.1383177E-522.069922
TGTAGGG9950.021.1351572
TGTATAG9300.021.0528722
GTGTACA10800.020.814621
TTAGCGG2455.1079805E-520.71875
Produced by FastQC (version 0.11.5)