FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1796_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1796_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298516
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC2650.043.77299145
GCGAAAG5700.034.348252
AGCGAAA5900.033.1839071
GCACCGT6000.032.63216
CACGAGC3450.031.521313145
AGCACCG6400.030.592595
CGTAGAC1000.006069804529.0051923
TCTCCGC4250.028.999607145
GTCTACG1306.477216E-427.8896081
CGAAAGC7200.027.1923683
ACCGTGT8050.026.119678
CACCGTG9150.025.3558257
ACATAGC10200.024.8819051
GGTAGTG6500.023.4236627
CCGTGTT9350.022.4880589
ATAGCAC11550.021.973633
ATGCGCC1650.002600897621.9702457
TAGCACC10300.021.1202874
TAGTGCA8600.021.076929
TAGTACC6600.020.8749474
Produced by FastQC (version 0.11.5)