FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1796_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1796_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127400
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5550.044.421871
AGCGAAA4850.040.3676031
ACGTCCG1901.2914825E-1038.157539
CGAGAGT1209.553925E-636.256094
TAGAGCG1301.6530663E-533.467165
CGAAAGC7700.032.0183642
ATATCGA1000.006069797629.0048736
ACCGTAC1450.001225340425.0041961
TAGTACC4500.024.1707254
CTAGTAC3452.9594958E-923.1198253
CACGAGC2202.2292941E-523.067963145
ACCGTTC2603.2050284E-622.3074828
CCGAGAG2303.1384086E-522.0689223
ACACCGT3406.421942E-821.327116
CCACGAC1750.00365921620.71409145
TTCGAAA1800.00430764220.138699145
GTACCTA5800.020.0033576
TCCGAGA2256.860664E-419.336582
TCGATTA3451.74257E-618.912865145
TAGTACT5901.2732926E-1118.4352994
Produced by FastQC (version 0.11.5)