FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1796_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1796_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682579
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6050.032.358091
GACTCGC1504.400624E-528.999851145
TCGCGTT1555.5028155E-528.064371145
GTCTACG1306.481617E-427.886961
CGAAAGC6300.027.6213672
AACCGGC1050.007710116427.618904145
CTAGTAC6850.027.5205613
GTATTAG9650.027.0489031
CCGACTA2507.831113E-826.1021924
ATACCCG2259.107025E-725.7799455
CTCCGAC1751.2562677E-424.857014145
ACCGTAG1851.8310844E-423.5140918
ACGTCCG1550.001808741423.387679
TACTGCG2502.2790937E-623.2019485
CCGAATA2852.7804708E-722.89539
TACTGTG19200.022.6568077
GCACCGT6800.022.3915886
GCAGTGC11450.022.1634351
GCCGAGT2303.14307E-522.0657628
AGCACCG6650.021.8063435
Produced by FastQC (version 0.11.5)