FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1796_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1796_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11399471
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC31250.042.22355145
TGATCCG30550.042.0059369
CGAAAGC59900.034.8650673
TCTCCGC43900.032.368675145
CTCCGAC16150.032.321636145
CATAGTG53800.024.935394
GTATATA54600.021.9135281
GTGATCG9000.021.7505329
GATCGAT37050.021.3300867
CTTAGAC59250.020.683463
GTCATAG68400.020.5669772
AGCGAAA106100.020.1617221
GTAGTAT60200.019.9955181
CCACGAC21800.019.62135145
TAGTATG46300.019.5773492
GATGCGC54800.019.4486378
GTGATCC73450.019.1497178
TATGCTG95950.019.1211365
TATAGAC32800.019.0129452
GCATCGA51250.018.814434145
Produced by FastQC (version 0.11.5)