FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1796_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1796_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203230
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC1751.8189894E-1245.570885145
AGCGAAA12600.039.134511
GCGAAAG13500.036.5272062
CGAAAGC15300.032.2298853
CGTTATA2151.8091669E-830.3546751
CACGAGC6050.026.363321145
TGAGCGC11900.026.197166145
TGATCCG3607.2759576E-1226.1808389
GTAGCGA1450.001225050325.0065146
TATACTG11950.022.4530375
GTATTCG2404.359478E-521.14991
GTACCTA8750.019.8908966
TAGACAG13900.019.8248585
TATAACG2205.897006E-419.777433
GTATTAT23000.019.2319581
TACGCCG1900.00588200419.0834835
TATTCGT2307.9533726E-418.917542
TAGTACC18450.018.8662744
TTAGACA10450.018.7365134
GAGCGAA4705.131369E-918.51421
Produced by FastQC (version 0.11.5)