FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1795_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1795_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822411
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8100.041.1825261
GCGAAAG8400.039.7117232
CGAAAGC13250.026.2703293
TCTCCGC8950.025.111439145
GACGTAG1450.001224953425.0065676
GTCCTAT13700.023.8194711
GAGCGAA2802.3394932E-723.3090531
CTCCGAC2953.878431E-722.118383145
CCTATAC7600.021.9459513
GTATAAA17200.020.658931
GCTATAT6250.019.724641
GCACCGT10100.019.386286
ACACCGT5305.638867E-1119.1559736
CGCTGCC7150.018.251534145
AGACGTA2400.001058297818.1297635
AGCACCG11400.017.8116955
CCCGCGT2050.00912982317.682718145
CCGTTCA3302.513392E-517.5760339
TACACCG3753.856696E-617.4045725
GTATAGA16350.017.2976461
Produced by FastQC (version 0.11.5)