FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1795_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1795_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545083
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC400.006049506454.374797
GTGATCG1209.556121E-636.2498639
TTAGCCG850.002740947334.11752145
GCGAAAG1751.00697434E-733.145772
CGATGCT1551.4634315E-632.741819
CTAGTAC2101.4360921E-831.0741623
ACGATGC2054.0335908E-728.2925768
ACGGGAC1050.00770211827.6214773
TCTCGTT1050.007705567427.618944145
TAGTACC2654.5492925E-927.3583899
TACACGA1606.818448E-527.189895
TACTGCG1100.00966797726.3635377
CCCACGC1409.988965E-425.892757145
CTACACG1701.0298339E-425.5904854
ACACGAT1751.2539522E-424.859336
CTATATC1751.2539522E-424.859334
CACGATG1751.2547337E-424.8570487
GTGTAGA2401.5946353E-624.168791
CGAAAGC2451.9079635E-623.6755523
AGCGAAA2202.2248825E-523.0702111
Produced by FastQC (version 0.11.5)