FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1795_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1795_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495074
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4500.048.3410031
GCGAAAG5250.041.435152
TATACGG800.002035350836.2557535
CGTAGAC1051.8524258E-434.5292853
CCGCGTG900.003628953132.2219479
CTCCGAC1601.8780647E-631.718481145
CGAAAGC6700.031.3855783
TCTCGTT4450.030.95479145
AAGATCG2207.4797754E-726.367825
CCGTAAC1409.997413E-425.8926359
AGATCGT2001.0646174E-525.3790286
TCTCCGC6250.023.199802145
ATCGTGC2852.7809438E-722.894548
TTATACG1600.002173644322.6598454
CCGACTA1600.002175758122.6560579
GTGTAGG7050.021.599171
ACTAACG2704.447622E-621.4812989
CACGAGC3406.4374035E-821.323349145
TAGTACC4801.4551915E-1121.149194
CGTCTTG4652.055458E-1020.268644145
Produced by FastQC (version 0.11.5)