FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1795_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1795_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932667
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC38040.19682645794645431No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC27100.14022074159697454No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT21660.11207310933544165No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC19650.1016729731505738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1354.973299E-737.5938389
CCACGAC3300.035.150856145
TCTCGTT7550.028.80741145
GTATAAG6600.028.571161
TCTCCGC7200.028.193916145
CGAAAGC6500.026.7791213
GTTATAT13150.026.4736561
GGTCGTT1450.001226806425.000187
TATACTG12250.024.8663275
GATATAT9850.024.2983151
CGTTTAT3702.6011548E-1023.5222021
TCGCGTT3702.6011548E-1023.513073145
TATAGAC8950.022.6899452
GCCGTCG3304.6966306E-821.969286145
GAATAAC14300.021.8087541
GCACCGT9150.021.4013466
GTAATAT8850.021.3073061
TAATACG2053.6590087E-421.2273524
ATAGCCC9600.021.146538
TTATATA18600.021.0561642
Produced by FastQC (version 0.11.5)