FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1795_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1795_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244018
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGC9050.036.04916145
GCGAAAG8450.033.4699632
CACGAGC7250.030.999516145
CGAAAGC10600.026.6812423
GCGATTA2051.2913528E-524.755709145
AGCGAAA11750.024.0688151
TACACCG3753.074092E-1023.2058415
TATACTG13950.022.3532965
GTGATCG2303.1430274E-522.0663479
GTCCTAT14500.020.5042481
AGAACCG3208.459865E-720.395765
GTATAGA22150.019.6428951
ATGCCGG7200.019.131643145
GACATCG10350.018.912746145
GTATTAT25250.018.3800451
GCACCGT5158.021743E-1018.3059866
ACCGTAC2000.00791571418.1263318
ATCGCGG1600.004450637418.124716145
GTATATA13500.017.7258951
TATAATA22150.017.6793922
Produced by FastQC (version 0.11.5)