FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1795_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1795_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531585
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG451.1941097E-464.4439145
GCACCGT4550.027.0929936
GATAGCG2156.128612E-726.976519145
CCGACTA2507.8222E-826.1048954
TAGTCGG1958.756806E-626.0262748
TCTCCGC5300.025.99035145
ACGGTAC1450.001225633925.0038721
GCGAAAG5300.023.2583221
TATACTG7900.022.9473425
CACCGTG7700.022.5979527
AGCACCG5550.022.2113725
GTTACGT2303.1396718E-522.0686361
TCGATTA6650.021.804329145
TACACCG3406.424307E-821.3275285
CGAAAGC5850.021.0723292
CTAATAC8950.021.0654033
GTGATCG2104.315817E-420.7141159
AGTCCGC2556.961017E-519.901794145
GGTCCTA12250.019.5304499
TATAGAC5254.9112714E-1119.336962
Produced by FastQC (version 0.11.5)