FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1794_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1794_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053027
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT701.6942204E-551.7951471
GACCGGC1703.8198777E-1142.646725145
TACACCG951.02782535E-438.1648455
GTTGACA9300.037.426171
ACATGGT9650.036.0687455
GCGATTA1153.1623585E-431.521492145
AACGGGT1402.7433973E-531.0770873
CCGACTA3350.030.3040264
TTACGTG1204.0538644E-430.2138332
ACGAACC3850.030.1353575
CCACGAA4150.029.70423
GCAGTGC7100.029.6180691
GAGCGAA1804.69362E-628.1995771
TTGACAT12350.028.1832682
GACATGG12100.028.1662864
ACGACTC1306.47628E-427.8896925
CAGTGCA7650.027.4886652
CTACTGA14650.026.7284156
AGTCCGC1658.41944E-526.363428145
ATGCCGG2207.487124E-726.363428145
Produced by FastQC (version 0.11.5)