FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1794_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1794_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164955
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14690.12609929138893777No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13350.11459670116013064No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA12960.11124893236219426No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA11770.10103394551720882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA8100.048.3471571
GGATAGC3800.043.894135
GATAGCG1451.9286745E-839.99942145
ACATGGT9850.039.021315
AGTCCGC2700.034.906902145
TTACGTG1051.8507898E-434.5336882
TCGCCAT3650.033.766632145
CGCATGT1801.2894634E-732.2314385
CACGAGC2051.1390512E-831.828806145
GACATGG12900.029.7953414
GCGAAAG4100.028.3007762
GCAGTGC7250.028.0080091
GTTATAG1606.815803E-527.1952761
CAGTGCA7750.027.1367912
TTGACAT14500.027.0077232
GGGTTAC1907.1408776E-626.7181661
TACGTTC1907.1408776E-626.7181664
GGTTCGA1907.157567E-626.7101388
GGTCCTA16850.026.6761729
ATCTATG5550.026.1335981
Produced by FastQC (version 0.11.5)