FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1794_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1794_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315638
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC53150.40398650692667737No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG40460.3075314030151151No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG39820.30266684300696695No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA37410.28434873422628415No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC32600.24778852541504576No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC31840.24201186040536987No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC31750.24132778165422403No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT29490.2241498041254509No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA28340.21540879786080971No Hit
GGTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCT25690.19526647907707134No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG24300.18470126280937463No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG23220.17649231779562463No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC22090.16790332903123809No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC21660.16463495277576354No Hit
TGGTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCC20180.15338565775692098No Hit
ATGTAGTACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC20120.1529296052561571No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT20090.15270157900577513No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC19920.1514094302536108No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT19680.14958522025055523No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT19660.14943320275030061No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT19150.1455567564938076No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC18920.14380855524087935No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG18900.1436565377406247No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA17520.1331673302230553No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG17490.13293930397267334No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT17250.13111509396961776No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG16910.1285307964652891No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG16890.12837877896503444No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16000.12161400020370344No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC15920.12100593020268494No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT15580.11842163269835623No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT15320.11644540519504605No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG14980.11386110769071736No Hit
TGCTAGTACTACTTGAAAATCAAAAAACACTCGATGAGCATGATGCGAAC14710.11180887143727987No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC14600.1109727751858794No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA14480.11006067018435163No Hit
ATAATAAGATTGATGACCAAATACAAGACGTATGGGCATATAATGCAGAA14210.10800843393091414No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT14140.10747637268002293No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA13860.10534812767645811No Hit
TGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATTG13850.10527211892633079No Hit
GTACTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG13800.10489207517569424No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA13790.10481606642556691No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA13790.10481606642556691No Hit
ATCTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTG13590.10329589142302062No Hit
CCATTGGACATGGCCCAGAACAGGAAGGCAGCAAACCCCATTGCAAGCAC13390.10177571642047434No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT13290.10101562891920118No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA5400.0108.7655942
GACTTAA404.816684E-9108.749054145
GGTACTG5200.0107.3752445
GTACTGA6900.088.2768866
GGGTAGA259.38065E-487.0157856
GCGAAAG2200.085.69412
AACACTG7100.079.662345
AGGTACT7250.078.0111854
CAGGTAC7600.074.4185643
GTTTATA300.001935069772.507641
TGGGCGG406.664255E-572.49937145
GGCAAAC1150.069.355137
AGTAGAA5850.068.169581
CGAAAGC2750.065.918543
CAGGGTG1051.6370905E-1162.154135
GAGCCCC1200.060.416145145
CACGAGC753.762343E-757.999496145
TTAAACC2400.057.404063
TCTATGT2150.057.33383
GGATAGC12000.056.801975
Produced by FastQC (version 0.11.5)