FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1794_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1794_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292540
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG218710.9540073455643086No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC210690.9190243136433826No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG162940.7107400525181676No Hit
GTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA129930.5667512889633333No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG106580.46489919477958946No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT88900.38777949348757274No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA86190.3759585437985815No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT77310.33722421419037396No Hit
GAATATAGGCTGTTGAACACCGTCAATGAAATGGAAGTCTGAATACATGA68620.2993186596526124No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA67930.2963088975546774No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA66150.2885445837368159No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG64510.2813909462866515No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG63590.27737793015607143No Hit
CACTTAGAAGTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAA62920.274455407539236No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC53480.2332783724602406No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA50430.2199743515925567No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC49910.21770612508396797No Hit
GTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGGTGTTCAACAGCC49580.21626667364582516No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT45670.19921135509085994No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG45610.19894963664756124No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA45530.19860067872316295No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT43220.18852451865616304No Hit
TTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACA42150.18385720641733622No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA41170.17958247184345746No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG40700.17753234403761767No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA39020.17020422762525408No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG39010.1701606078847043No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT38710.16885201566821081No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA38620.16845943800326274No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG38200.16662740890017186No Hit
GCAATATGCACACACTTAGAAGTCTATATTCATCTCCACAACTCGAAGGA37960.16558053512697707No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG37780.16479537979708095No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC37690.1644028021321329No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT37600.16401022446718486No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC37490.16353040732113724No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG37050.16161113873694677No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG37030.16152389925584723No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA36550.15943015170945762No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT36250.15812155949296414No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC36240.15807793975241435No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA35190.15349786699468712No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT34490.1504444851562023No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC33480.1460388913606742No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGGTGT33300.1452537360307781No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT33040.1441196227764837No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT32020.13967040924040583No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG32000.13958316975930626No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC30830.13447966011498164No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG30610.1335200258228864No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC30060.13112094009264832No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA29970.13072836242770028No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA29730.1296814886545055No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC29600.12911443202735828No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC28920.12614828966997305No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGA28530.1244471197885315No Hit
GTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATGAA28360.12370558419918518No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT28360.12370558419918518No Hit
ACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG27810.1213064984689471No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA27730.12095754054454884No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG27500.11995428651190383No Hit
ATAATAAGATTGATGACCAAATACAAGACGTATGGGCATATAATGCAGAA27360.11934361014420687No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA27290.11903827196035838No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG27280.1189946522198086No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT26950.11755520078166576No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG26840.11707538363561815No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA26790.1168572849328692No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT26510.11563593219747528No Hit
CTTAGAAGTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATC26350.11493801634867877No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT26330.11485077686757919No Hit
ATACAAGACGTATGGGCATATAATGCAGAATTGCTAGTACTACTTGAAAA25940.11314960698613766No Hit
GTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCG25510.11127395814249698No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG25500.11123033840194718No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC25180.10983450670435412No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC24720.1078279986390641No Hit
CTGCAATATGCACACACTTAGAAGTCTATATTCATCTCCACAACTCGAAG24620.10739180123356626No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC23070.10063074144834987No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGC100.0070879324145.024112
GCAGGTA26950.0118.117972
GGTACTG28650.0110.856125
GACTTAA553.6379788E-12105.453674145
CAGGTAC32400.098.025553
GTACTGA34100.094.627166
CCACGAC1150.094.56443145
AGTAGAA15350.090.699111
CCCACGC1950.085.51211145
TTTCGTT554.397225E-879.090256145
CTCCGAC950.076.315155145
AGGTACT44100.074.8140264
CTGGCCG300.001936126472.4994145
AGCAGGT47000.067.729341
AACACTG8650.066.2248845
CTTATAA2550.065.39162145
CACGAGC2750.063.272205145
GAGCCCC1950.063.204605145
ACTGATC62350.058.487898
TTTTTGG39550.058.1196481
Produced by FastQC (version 0.11.5)