FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1794_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1794_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102489
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG114080.542594990984495No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC31770.15110661696684263No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG31390.14929923533488165No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT30950.14720647765576894No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT27940.132890112623657No Hit
GTTTAGAAATGTCTTGAGCATTGCCCCTATAATGTTCTCAAATAAAATGG25100.11938231305847496No Hit
CTTATTAGGTAGCTCTTTTTTGTCAGCTTTTGCTTCTTCTTCCCTATTGT23770.11305647734661158No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG23320.11091615699297355No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT23050.10963196478079076No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA21780.10359150511607908No Hit
CCATAGAGGTATTCAGATCGAACGGTCTAACAGCCAATGAGTCAGGAAGG21310.10135605941339051No Hit
CCTTTACAGTTACCGGAGACAACACCAAATGGAATGAGAATCAGAATCCT21180.10073774464456176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG7250.066.0149155
TCGCGTT753.7635255E-757.999306145
GCAGGTA8600.053.1224632
TATAGCG7950.048.3442542
AGGTACT10700.048.1183434
GTACTGA10600.047.8870356
CTATTAG37700.043.086651
TCGCCAT35800.040.90733145
TATTAGT40200.040.407142
TAGTAGA39850.040.2161065
GGATAGC10400.039.7445535
CAGGTAC11700.039.6670763
CGAAAGC5050.038.7711333
AGAGGTC42150.037.841499
ATTAGTA44500.036.6655853
ACTGATC18000.036.653218
GTATAGG7750.035.556421
GCGGTAT10950.035.098426
TTAGTAG47500.034.807864
GTATCCC12750.034.6866849
Produced by FastQC (version 0.11.5)