FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1794_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1794_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592421
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG26870.16873678505872505No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA19820.12446457312482062No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17270.1084512198721318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6000.058.0100252
CGAAAGC6800.052.251683
ATGCGCC1457.2759576E-1249.99922145
GCGGTAT2450.041.4383356
TATAGCG2450.041.4357342
TCGCCAT9200.036.249435145
CTCCGAC1656.0214006E-835.150967145
AGCGAAA9950.034.252151
GGTACTG6450.033.7278185
GTATAGG6950.033.3870661
ACTGATC12250.030.7779318
ATGCCGG1703.013558E-629.852478145
GCAGGTA6950.029.2136822
CCCACGC4100.028.292244145
TATACTA5400.028.2002055
CTATTAG15700.028.1736581
CCGAACT1555.4959633E-528.0693664
GTTCGTA1050.00771038827.618614145
AGGTACT8500.027.2988364
TCCGAAC1606.822604E-527.1921983
Produced by FastQC (version 0.11.5)