FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1793_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1793_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349519
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5200.048.7994581
CGAAAGC5700.043.2484442
AGCGAAA5850.028.5050811
TATACTG8500.027.2959045
TGATCCG1100.00967142426.3643849
GTACCGT2001.0652879E-525.3766613
CTCCGAC1450.001226727724.999784145
TATAGAC6150.024.7577152
GTGATCG2101.5552505E-524.1673539
TCTCGTT2451.913053E-623.673262145
GCACCGT8750.023.2015176
CACCGTG10950.023.1741717
TACCGTC1952.6142434E-422.309154
TACACCG2303.141485E-522.066665
AGCACCG9600.021.9024755
GTCCTAT10900.021.2850071
ACCGTGT10200.020.6140948
GTCTAAG6000.020.542251
TTACGTG1800.00430620920.1402052
GTTACGT1800.004307132720.139461
Produced by FastQC (version 0.11.5)