FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1793_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1793_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985839
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC24010.12090607546734655No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC20270.10207272593598979No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG750.0014823438.6740882
CTCCGAC2103.783498E-1034.523483145
TATACTG11850.034.268185
TCTCCGC7050.031.879128145
GCGAAAG7550.031.6948222
AGCGAAA8000.029.911991
CGAAAGC8650.027.6642673
ACTCCGC1358.084795E-426.8522728
AGATCCG1658.4116895E-526.3686945
TCTCGTT5500.026.363386145
ATGCCGG6300.025.317219145
GATAGTG7300.021.8535082
CACGAGC4006.91216E-1021.74979145
GCACCGT8700.021.6708266
GCCGTCG4109.404175E-1021.219309145
AGCACCG9250.021.1662245
CCACGAC2505.978739E-520.299805145
GTCCTAT18550.019.9364411
GGATAGT8800.019.7765241
TACGAGG2959.568719E-619.6613257
Produced by FastQC (version 0.11.5)