FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1793_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1793_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467062
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2700.053.714452
AGCGAAA2700.053.714451
CGAAAGC3400.042.6555943
CCCTACG1001.3904291E-436.2494938
ACTACGT1255.1501096E-428.999594145
CCTACGC1100.00966706926.3632689
CATAGGC1958.727873E-626.0308515
TTAGGGC1701.0287399E-425.5933573
ATTATAC2451.9051295E-623.6782071
GCACCGT1851.826154E-423.5182196
GGTATAC1550.001804950223.3917771
TCTAGAG2552.6972648E-622.749653
GTATTAG2003.0954226E-421.7543531
GCCCTAC1750.003656150520.7139957
GTCCTAT3208.4346175E-720.3947071
TAGTATA1900.00587649219.0827662
GACCTAG1900.005883767319.0786787
GTCATAG2307.9425663E-418.916831
GTGTAGG2307.9425663E-418.916831
TATGCCG3504.0017767E-1118.642595145
Produced by FastQC (version 0.11.5)