FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1793_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1793_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920671
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG7950.040.13782
TGATCCG750.001483822238.666169
CGAAAGC8150.038.2629853
TATAGCG1354.9629125E-737.6038482
CACGAGC4850.034.380993145
AGCGAAA9200.033.895131
TCGCCAT6150.027.113468145
TAACGTA5050.024.4132485
CGCTATA1500.001492169524.1739032
CTCCGAC2502.2812965E-623.199696145
GACATCG6250.023.199696145
GGTCGTT3803.6379788E-1022.8950357
ACACGAC1902.1943849E-422.894438145
TATACTG11350.022.3635225
ATGCCGG3055.346119E-721.393162145
TACACCG3406.41885E-821.3299145
GTACGGG1700.003088918521.329361
TATGGCG2053.6684633E-421.219233145
ACCGTTC2404.3651366E-521.1461058
CTATAGG6500.020.082411
Produced by FastQC (version 0.11.5)