FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1793_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1793_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1845756
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC19830.10743565238308857No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG1051.6370905E-1162.14244145
TATAGCG701.694986E-551.7937852
ATACCGT1255.1508076E-429.0045196
GCGAAAG6700.028.1387122
AGTCCGC2954.838512E-1027.033718145
ATAGTCG2456.445043E-826.6324758
GGATAGC5200.026.4945135
ACGGACT2207.481558E-726.3677445
GCACCGT12250.024.8610156
CGAAAGC7950.024.626483
CTTAACG2101.5541176E-524.1704331
ACGTCCG1801.5210346E-424.1665049
AGCACCG13700.022.7590225
AGCGAAA7900.022.0287481
GTTACGT1650.002599332521.9731221
TATACTG12650.021.7820515
ACCGTGT14400.021.649168
CACGAGC3809.056748E-920.986702145
TCGACAC2455.110872E-520.7175143
CCGTACA1750.003656583820.7175122
Produced by FastQC (version 0.11.5)