FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1793_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1793_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233669
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC13180.1068357882057505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5100.041.2398342
GCGAAAG5350.039.3127371
TCGCCAT4650.034.30049145
CGACAGT2251.0913936E-1132.2347376
CCGACTA1652.3805605E-630.7682724
GTTGACA11600.030.0104351
CGCTGCC3700.029.391392145
GGTCCTA12650.028.65689
TATACTG10450.027.7619745
ACATGGT12350.027.0145365
CTCCGAC1100.00967324626.363188145
AGTCCGC3057.1122486E-1026.147095145
TCTCCGC4200.025.892418145
AACGAGG3108.58563E-1025.725368145
GACATGG13150.024.8185184
CCCACGC3251.4861143E-924.538044145
GGTACTG5400.024.1760525
GTCGTAA600.00470622624.1750741
CACGAAC3952.3646862E-1123.869064
ATCTATG7000.023.8297141
Produced by FastQC (version 0.11.5)