FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1790_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1790_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321709
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17990.13611165544004014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG2250.048.33293145
CGGAGCG450.00964013548.33293145
AGACGGC2000.043.499638145
GCGAAAG4950.041.0144162
TCTCGTT3200.031.718487145
CGAAAGC6500.031.2340553
TACATCG1703.0128776E-629.852692145
AGCGAAA7350.028.6084521
TAGTGCA9200.026.0071859
CACGAGC2651.3771387E-724.622437145
GAGCGAA1902.1921267E-422.8971441
CCCACGC2852.7804163E-722.894547145
TATACTG9250.022.7321195
GTATACG1600.002174188222.6586341
GGTAGTG8850.022.1192977
TCTCCGC3355.502625E-821.641611145
CTTAGAC5550.020.90313
GAGGTCG2205.9035706E-419.773318
GTCCTAT12900.019.6726111
TAGACAG10900.019.2910185
Produced by FastQC (version 0.11.5)