FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1790_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1790_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372779
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG2800.033.66041145
GCGAAAG6450.030.3551961
AGCGAAA6400.027.1931971
GACTCGC1701.03163016E-425.588005145
TACACCG3451.07320375E-1025.2226755
CGAAAGC7650.024.6456872
GTCCTAG11600.023.7549761
CACGAGC3554.129106E-922.464586145
ACACCGA2303.1380434E-522.0698416
GTGTAGG10700.021.6867871
TACACAG16600.020.9682485
GTAGGAC9200.020.4934233
CCTAGAC9250.020.382653
TAGGACT11200.020.0711694
GACGTCC3701.5710248E-719.594418145
TAGGCTG5605.456968E-1219.4237125
AGGTAGT7600.019.0829456
TACACCA10500.018.6467655
CCTAGCT5458.54925E-1118.6277563
GTCCTAT9050.018.4292761
Produced by FastQC (version 0.11.5)