FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1790_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1790_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866342
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3500.041.4353332
GTGATCG1252.717352E-740.601949
CGAAAGC3800.036.2559173
AGCGAAA4250.034.1232151
TCTCCGC3057.1122486E-1026.14728145
AGCGGTC1500.001493696824.167827
GTATAGA7300.023.8395061
ACGTCCG2252.650035E-522.5566339
TAGTACC4307.094059E-1121.9234494
TATACTG5400.021.4862275
GTGTAGG4851.6370905E-1120.9312521
TACTAGC3208.4547537E-720.3939532
ACACCGT2505.9631708E-520.3044856
TCGATTA3951.4113539E-820.18967145
GTATACC1800.00430254820.1421763
CCTATAC2908.235773E-620.0032633
TATAGAC3351.3119425E-619.4807912
TATAACG2256.8564585E-419.3376055
GTATAAA9850.019.1401791
CTAATAC4654.485628E-918.7127323
Produced by FastQC (version 0.11.5)