FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1790_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1790_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381123
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGAGTGGTTACAAGTTGGCCTGAGTGCCAGAGGGCAGCAGGGAAG4380.11492352862461726No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG350.003561258362.1584935
AGATCGT400.006048283554.374417
GAGCGAA858.894658E-751.1893461
TTGCCGC450.00963500848.33281145
GAGATCG700.001054510841.4389956
GCAGTGC2550.039.813941
CTAGTAC1153.1537973E-431.5296733
GCGAAAG1402.7375425E-531.0792471
CAGTGCA3450.029.427692
GTACAGC1000.006062347429.0072966
AGCGAAA1555.4822136E-528.0715752
GTGGTTA2654.5401976E-927.3581929
AGTGCAG3750.027.0734793
CTTAGAC1907.1262893E-626.7172473
AGGAGTG3357.4578566E-1125.9766835
GAGTGGT3108.54925E-1025.7255277
GGTTGTC4600.025.2171177
GTGCAGC4051.8189894E-1225.0680354
CTATACT1500.001490394124.1727474
TCTAGGA4303.6379788E-1223.610592
Produced by FastQC (version 0.11.5)