FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1790_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1790_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826357
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGC858.9162495E-751.1869965
GCGAAAG10050.038.2406462
AGCGAAA10200.037.677251
ATGCGCC2700.037.59209145
ACCGTAT1251.2650629E-534.8023958
TCTCCGC8100.033.11684145
CGAAAGC12100.031.761863
ATACCGC1204.0544593E-430.2145466
GTATAAG5550.028.7430741
GTACGCT1555.4951706E-528.0702886
ATGCCGG3800.026.710169145
TACCGTA1658.4199426E-526.3647277
AATACCG2507.822564E-826.105375
GTATTAG8650.025.1483041
CACGAGC5950.024.36942145
CCGTCCG1500.001494385324.1676659
AGTTGCG1550.001808623723.3880637
CTCCGAC2502.2812E-623.19969145
GCGCAGC10200.022.744793145
TATACTA5101.8189894E-1221.3279155
Produced by FastQC (version 0.11.5)