FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1790_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1790_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911599
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5350.075.891442
AGCGAAA5750.070.612031
CGTAGAC606.8032077E-660.419523
CGAAAGC6850.059.2728773
GTATACG950.00472598530.5277611
GACCGGC2003.2540265E-728.99978145
CGTAAGA1306.480714E-427.8859332
GCGGATA1050.007706321327.6203542
TATCGAG1050.007706321327.6203541
GCGTAAG1050.007706321327.6203541
CCGGTAA1050.00770838427.61884145
ACGTAGC1358.079928E-426.8531235
TAGAGCG1658.415061E-526.3648837
TCTACGT1658.415061E-526.3648832
GTCCTAT4700.026.2246461
CTCTACG1701.03086306E-425.5894471
TCCTATG5100.025.5894452
TGATCCG1450.001226021725.0011839
CGTAGCT1450.001226021725.0011836
GGAACGC2751.9683648E-723.727095145
Produced by FastQC (version 0.11.5)