FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1789_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1789_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233811
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC16930.13721712644805403No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT14370.11646840561479839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG2200.049.435139
TCGTCCG450.00963711448.3365759
CGCTATA657.328973E-444.625612
ATGCCGG2400.042.291077145
GCACCGT6950.041.7378736
AGCACCG7800.037.1880075
CACCGTG9250.034.48747
ACCGTGT9100.032.6670278
ATCGTCC1453.4894525E-530.0020128
CCGTGTT10250.029.7093099
TATAGAC4650.029.6304472
CTCCGAC1255.1549514E-428.999596145
ATAGCAC11950.028.521183
CCACGAC1555.501976E-528.064121145
TCTCGTT2853.237801E-1027.982063145
TAGCACC11450.027.866654
ATCGTCG1050.00770661527.62098
AGTGATC6350.027.4034128
GCGAAAG2954.820322E-1027.0400942
TATACCG1358.0729736E-426.8580065
Produced by FastQC (version 0.11.5)