FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1789_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1789_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643749
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG11350.036.4195252
CGAAAGC12350.033.4705773
AGCGAAA12350.033.4699441
TCTCCGC10300.028.154953145
TCTCGTT11700.024.785986145
CGAGACG2101.5564909E-524.166336145
CACGAGC5900.023.347137145
TATAGAC13100.023.2505512
TATACTG18950.021.8132785
GTGATCG4701.0913936E-1121.595459
GTATTAG12400.020.468851
ATGCCGG10350.019.613258145
GTATACG4452.6029738E-919.5554711
ATCGCGG4152.4951078E-819.216604145
GCACCGT16300.018.6860286
GTATATA22600.018.2899911
ATAGCAC22650.017.9297873
AGCACCG16800.017.698235
CTATACG2050.00912998717.6830188
TACTGTG25500.017.6275427
Produced by FastQC (version 0.11.5)