FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1789_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1789_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339660
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT20480.15287460997566546No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC17510.13070480569696788No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC13930.1039816072734873No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGT400.00604989154.3782779
GACCGGC1900.053.420284145
ACTACGC2050.042.44951
GCGAAAG5800.041.2601852
AGCGAAA5900.040.560861
CGAAAGC6500.036.816783
CTCGGCG850.002738998334.1260721
CTACGCA2353.45608E-1133.944552
CACGAGC3300.032.95407145
GCGTAGT900.003624283532.230183
ACGACTC1352.139127E-532.230185
TGCGTAG1153.1580921E-431.5295242
CGGCGTA950.004722155630.5338553
GTCGAAG950.004722155630.5338553
ATGCCGG2151.8117134E-830.3484145
TCGGCGT1000.006067860429.007162
GGTAGTG6500.029.0017477
TATACTG7450.028.2284455
CTACTGA14050.027.357556
GCAGTGC7050.026.744191
Produced by FastQC (version 0.11.5)