FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1789_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1789_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142410
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC49900.23291526831932266No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT48040.22423345671463446No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC30640.14301650944497085No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT28890.13484813831152767No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA28010.13074061454156768No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC26920.12565288623559448No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC26150.1220588029368795No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA26080.12173206809154176No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA26060.12163871527858813No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC24320.11351702055162177No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT21760.10156786049355632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG1700.055.4582372
GTTACGT1850.050.961621
CTCCGAC1203.6252459E-948.332413145
AAGGTCG4300.047.2117089
TAGCGCG2109.094947E-1237.975464145
GTAGAAA16150.037.2715262
ACGTGTT2700.034.9181484
CGGAATT1051.850818E-434.535246
AGCGAAA8950.030.7915951
CACGAGC3550.030.633217145
TATACTG20400.030.5738415
TATAGAC10150.030.009232
ACTACGC2701.70985E-1029.5461241
GCGAAAG9250.029.0089232
GCGTAGT1504.3918008E-529.0089233
GTCGTCG1000.006076554328.999445145
CGAAAGC9700.028.4108033
CGTCCAG1855.8052465E-627.4415135
ACAAGGC16650.026.9983718
GTTGACA10550.026.8091951
Produced by FastQC (version 0.11.5)