FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1788_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1788_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926564
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1553.466448E-837.4290285
TGATCCG6450.030.3504569
ACGGACT4550.027.0949155
ACCGCGT2001.0659423E-525.3763548
GACGTCC8300.025.330948145
CGACGAT1450.001225161225.006466
TCTCCGC13650.024.43187145
CTACGGA4951.8189894E-1221.9746253
AGCGAAA21600.021.821651
CGAAAGC22800.020.9915493
GTATACG6450.020.236751
GCATCGA6850.020.109188145
TCTACGG6150.020.0451452
TAGGACT24250.019.437684
TACGACG2659.340199E-519.1555654
GTATATA19350.019.1124861
TATAATA18600.019.1037462
TATATCG1900.005881752419.083885
TATACTG12950.019.0396695
GCGAAAG25050.018.8165952
Produced by FastQC (version 0.11.5)