FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1788_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1788_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757628
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAAT91780.5221810303431671No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG84710.48195636391773455No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC80490.4579467327557367No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA71140.40475003811955657No Hit
GTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGGCGTTG70470.40093808246113516No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT69130.39331417114429224No Hit
GTCTTCCGACTGGGCTCTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT57970.32981950674431676No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG53210.30273755311135236No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGA52660.29960833577981233No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG51970.29568259040024397No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA47660.271160905493085No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGC44970.2558561879988257No Hit
CTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG43540.24772022293682167No Hit
GTCCTGTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTC42700.24294105464865148No Hit
CCCATATACAGCGCATCTGGATGCACCAGACGGCAGTCGCGCATGAAGAT40670.2313913979522402No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG40120.22826218062070017No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG39800.22644154508234962No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG38880.2212072179095918No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT38470.21887452862608012No Hit
GGTCAGAACAGGACGTGTTGATAAAACAGAATTTGCCTGGCGGCCGTAGC38390.21841936974149248No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA36790.20931619204973975No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT35920.2043663391798492No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC35810.20374049571354122No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA35760.20345602141067393No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG35380.20129401670888267No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA34100.19401147455548046No Hit
GTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACA32810.1866720375415048No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC32390.1842824533974197No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA32160.18297387160423026No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT32070.18246181785906915No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT31380.1785360724795008No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG30920.17591890889312187No Hit
TTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCA30870.1756344345902546No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG30260.17216384809527385No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG29740.16920531534545422No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG29740.16920531534545422No Hit
CCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCCTCA29580.16829499757627894No Hit
GTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG29430.16744157466767712No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA29140.16579162371104694No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT28590.1626624063795069No Hit
GTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGC28100.15987455821140764No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAC27670.1574280792067491No Hit
CTTCAGGACCCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCG27240.15498160020209054No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT27170.15458333617807637No Hit
GGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT27050.1539005978511949No Hit
CTCCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG26820.15259201605800546No Hit
CCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGG26540.1509989599619487No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA26400.15020243191392035No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG26280.14951969358703887No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT26260.14940590386589198No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA26250.14934900900531853No Hit
GATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATT26150.148780060399584No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA25430.14468363043829524No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT25270.14377331266911997No Hit
ATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAC24960.14200957199134287No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC24770.14092856964044725No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC24550.13967688270783124No Hit
CCCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGA24300.13825451119349488No Hit
GCCTGATACAGATTAAATCGACAGGTCAGAACAGGACGTGTTGATAAAAC24130.13728729856374614No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG23120.13154091764582723No Hit
AAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGC22970.1306874947372254No Hit
GCATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCTCT22530.12818412087199338No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT22350.1271600133816712No Hit
CTCCCTGACTCCTTGAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTG22020.1252824829827472No Hit
AGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT21700.12346184744439666No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT21500.12232395023292755No Hit
GCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATC21410.12181189648776648No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG21080.11993436608884246No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT20810.1183982048533592No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG20800.11834130999278573No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC20770.11817062541106538No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC20760.11811373055049192No Hit
GAACAGGACGTGTTGATAAAACAGAATTTGCCTGGCGGCCGTAGCGCGGT19930.11339145712289517No Hit
CTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAG19900.11322077254117481No Hit
GGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCAT19800.11265182393544027No Hit
TATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACCCG19780.11253803421429336No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA19780.11253803421429336No Hit
TTTAGCTACCGGGCAGTGCCATTGGCATGACAACCCGAACACCAGTGATG19770.1124811393537199No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCAC19690.11202598046913227No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTCAAGGAGTC19680.11196908560855881No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC19510.11100187297881008No Hit
GGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAACAC19080.10855539397415152No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT18940.10775886592612316No Hit
GATTAAATCGACAGGTCAGAACAGGACGTGTTGATAAAACAGAATTTGCC18850.10724681218096208No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC18780.10684854815694789No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG18490.1051985972003177No Hit
GCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAG18250.1038331205465548No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG18220.10366243596483442No Hit
CTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACC18180.1034348565225406No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGTGCGGCTGCGGCGTGG17810.10132974668132279No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA17760.10104527237845551No Hit
CATCAGGATCGATAAACAGCGCATCCATATGCTGCCCACTTAACAACATA17670.10053321863329441No Hit
CGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGG17660.10047632377272095No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTG14800.072.507814
GTCATAG14750.072.2620242
TCATAGT14750.071.278863
CGAAAGC14050.070.701563
GGTCATA16800.064.307521
TGATCCG19550.054.8861359
ATAGTGA19950.054.8805965
CGGTAGA3350.049.781481
TAGTGAT22750.048.1260646
GGACGGA8100.042.967596
GAGCGAA3050.042.7914921
AGTGATC25750.042.232757
GACGGAT8550.040.7015087
GTGATCC27300.039.8349238
AGCGAAA25700.038.3698921
GTTAAGG9300.038.203042
AGGACGG9350.037.2232635
GCCTATA1458.703828E-734.999786145
TAAGACG1707.821109E-834.1213234
CTCCGAC850.0027426334.11744145
Produced by FastQC (version 0.11.5)