FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1788_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1788_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559903
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6350.066.2285161
GCGAAAG8050.053.1430972
CGAAAGC9400.046.2822153
TATACTG11300.031.441875
CGCTGCC4150.029.698593145
TCGTTAT1753.7728314E-629.0035211
TACTCGG1358.0790214E-426.8551122
ATATGCG1100.00967353726.3634578
GTAGCAC5250.024.860163
ACGGTGT2451.9133404E-623.6733068
GTATGAG6900.023.1187481
CACGAGC4103.8198777E-1122.987648145
TCTCCGC6100.022.581812145
GAGCGAA2953.8727194E-722.1213281
GTGTAGA8550.019.5052911
AGACTGC9300.019.4916136
GTGTAGG12850.019.1852071
CGGCGTG1900.00588956619.078817145
GTATAGA17750.018.7910141
ATAAGAC5509.640644E-1118.4567873
Produced by FastQC (version 0.11.5)