FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n01_1788_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n01_1788_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324461
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4550.044.6249121
GGATAGC4650.043.6652375
GCGAAAG5000.040.608672
ACCAACG800.002036605736.25097
CCCGCGT1501.1340398E-633.834178
CCACGAC1751.0097392E-733.14243145
TCCCGCG1551.4651996E-632.7427447
CCGCGTG1352.1423608E-532.223029
GGTACTG7500.031.906815
TGGATAG6300.031.0780624
CGAAAGC7100.028.5976563
TATCCCG1804.693671E-628.2004645
ATGCCGG1306.484564E-427.88425145
CCGTTCA1701.0313668E-425.588879
CAGGTAC10550.024.744623
ACTGATC10500.024.1672658
GCAGGTA9400.023.143242
GTATGGA9500.022.1363051
CACGAGC3655.689799E-921.84903145
TCTCCGC4006.91216E-1021.749716145
Produced by FastQC (version 0.11.5)